■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，信號通路報告載體 |
質粒大小: | 6470bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃ |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | M13F：TGTAAAACGACGGCCAGT |
備注: | 信號報告空載體 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: | miRNA,UTR
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Puro
|
熒光標記: | Fluc
|
■ 質粒簡介
The pMIR-REPORT miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.
本質粒的參考文獻：Evaluation of the circulating MicroRNA‐495 and Stat3 as prognostic and predictive biomarkers for lower extremity deep venous thrombosis.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK492pMIR-Report Luciferase哺乳報告質粒.txt
