■ 基本信息
啟動子: | TK |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，信號通路報告載體 |
質粒大小: | 4045bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: | Fluc
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■ 質粒簡介
The pRL-TK Vector may be used in combination with any experimental reporter vector to cotransfect mammalian cells. However, it is important to realize that trans effects between promoters on cotransfected plasmids can potentially affect reporter gene expression. This is primarily of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements.The occurrence and magnitude of such effects will depend on several factors: i) the combination and activities of the genetic regulatory elements present on the cotransfected vectors, ii) the relative ratio of experimental vector to control vector introduced into the cells, and iii) the type of cell transfected.
本質粒的參考文獻：YAP1 negatively regulates chondrocyte differentiation partly by activating the β-catenin signaling pathway.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK487pRL-TK哺乳報告質粒.txt
